DNA Mixture Simulator
This simulator shows how two or three DNA profiles behave when mixed, in both capillary electrophoresis (CE) and NGS. It helps users understand how peak heights shift, how stutter artifacts appear, and how minor alleles can become hidden or confused with noise, illustrating the real complexity of interpreting mixed profiles in forensic genetics. Profiles are derived from open-access 1000 Genomes samples, except for the simulated triallelic pattern example.
True genotypes at D18S51
Contributor AHG02944
15, 16
Contributor BHG00097
14, 14
Contributor CNot selected
—
Contributor AHG02944
%
Contributor BHG00097
%
Contributor CNone
%
AT (RFU)
ST (RFU)
Degradation k (per 100 bp)
Noise / Base (RFU)
Stutter level (×)
Capillary Electrophoresis Analysis (RFU)
Next-Generation Sequencing Analysis
Individual haplotypes inferred from Illumina short-read NGS data using specialized STR genotyping software (HipSTR, hg38), displayed for educational purposes only.
| Allele | Allelic Coverage (PDP) | Repeat Sequence | Full Sequence |
|---|---|---|---|
| 14iso | 45.07 | [AGAA]14 | GAGTGACAAATTGAGACCTTGTCTCAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAGAGAGGAAAGAAAGAGAAAA |
| 14 | 3.93 | [AGAA]14 | GAGTGACAAATTGAGACCTTGTCTCAGAAAGAAATAAACAAATAAAGAAACAAACAAACAAAGAAAGAAACCAACAAAGAAAGAGAGAGGAAAGAAAGAGAAAA |
| 15 | 31.43 | [AGAA]15 | GAGTGACAAATTGAGACCTTGTCTCAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAGAGAGGAAAGAAAGAGAAAA |
| 16 | 30.57 | [AGAA]16 | GAGTGACAAATTGAGACCTTGTCTCAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAGAGAGGAAAGAAAGAGAAAA |