STRhub

DNA Mixture Simulator

This simulator shows how two or three DNA profiles behave when mixed, in both capillary electrophoresis (CE) and NGS. It helps users understand how peak heights shift, how stutter artifacts appear, and how minor alleles can become hidden or confused with noise, illustrating the real complexity of interpreting mixed profiles in forensic genetics. Profiles are derived from open-access 1000 Genomes samples. For educational purposes only, not validated for forensic casework interpretation.

True genotypes at vWA
Contributor AHG01063
14, 16
Contributor BHG00097
17, 17
Contributor CNot selected
—
Contributor AHG01063
%
Contributor BHG00097
%
Contributor CNone
%
AT (RFU)
ST (RFU)
Degradation k (per 100 bp)
Noise / Base (RFU)
Stutter level (×)

Capillary Electrophoresis Analysis (RFU)

Next-Generation Sequencing Analysis

Individual haplotypes inferred from Illumina short-read NGS data using specialized STR genotyping software (HipSTR, hg38), displayed for educational purposes only. Repeat-region sequences follow the ISFG STR sequence nomenclature recommendations (via STRNaming). Read the original paper.

Allele
PDP
Repeat Sequence
Full Sequence
1416.55
GGAT[3] AGAT[1] GGAT[1]
AGAT[8] AGAC[4] AGAT[2]
AATACATAGGATGGATGGATAGATGGATAGATAGATAGATAGATAGATAGATAGATAGATAGACAGACAGACAGACAGATAGATCAAT
1614.45
GGAT[3] AGAT[1] GGAT[1]
AGAT[10] AGAC[4] AGAT[2]
AATACATAGGATGGATGGATAGATGGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGACAGACAGACAGACAGATAGATCAAT
17iso18.99
GGAT[3] AGAT[1] GGAT[1]
AGAT[11] AGAC[4] AGAT[2]
AATACATAGGATGGATGGATAGATGGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGACAGACAGACAGACAGATAGATCAAT
1723.01
GGAT[3] AGAT[1] GGAT[1]
AGAT[12] AGAC[3] AGAT[2]
AATACATAGGATGGATGGATAGATGGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGACAGACAGACAGATAGATCAAT